The Impact of Microarray Image Intensity Variations on SNP Genotype Calls
Keywords:
Genotype, image processing, microarray, SNPAbstract
Microarray technology is widely used to determine
single nucleotide polymorphism (SNP) genotype calls when studying the association between disease and human subjects. A genotype call is decided relatively with the signal intensity values of spots on the microarray image. Since high-throughput microarray technology can contain millions of SNP spots, it is
impossible to manually inspect if the spots are aligned correctly and intensity values are calculated accurately. However, a wrong genotype call will skew the outputted numerical results, which can ultimately cause unsuitable medicine to be prescribed and jeopardize a person’s life. Therefore, retrieving correct intensity values is very important. This paper analyzes and shows the effect signals have on the SNP genotype calls when the
signals from the same spots are retrieved and represented in different values.
Downloads
Downloads
Published
Issue
Section
License
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
